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目的:了解不同负荷递增耐力训练方式对大鼠快、慢肌ATGL,HSL,CGI-58,Perilipin基因表达的影响。方法:通过跑台耐力训练构建低负荷递增耐力训练、中负荷递增耐力训练和高负荷3种运动训练方式。4周耐力训练结束后次日,宰杀大鼠。通过逆转录聚合酶链式反应测定腓肠肌、比目鱼肌ATGL,HSL,CGI-58,Perilipin基因表达。结果发现不同强度递增耐力训练方式引起大鼠腓肠肌和比目鱼肌ATGLmR-NA表达出现差异。中等负荷递增耐力训练时,腓肠肌和比目鱼肌中ATGLmRNA表达同时达到峰值(P<0.05)。在相同训练方式下,腓肠肌和比目鱼肌的ATGLmRNA表达差异并不存在统计学差异(P>0.05)。而HSLmRNA表达大负荷的递增耐力训练达到峰值,差异却不具有统计学意义(P>0.05),但在腓肠肌和比目鱼肌不存在差异(P>0.05)。腓肠肌和比目鱼肌在相同训练方式下,在最大负荷训练时腓肠肌CGI-58mRNA表达显著高于比目鱼肌(P<0.05)。而在不同强度递增耐力训练和不同肌肉之间,都不存在per-ilipinmRNA表达的差异(P>0.05)。结论:ATGLmRNA的表达对耐力训练的与负荷强度有关,而CGI-58mRNA表达的快、慢肌差异只会在高负荷耐力训练时出现;而HSLmRNA与PerilipinmRNA表达不受耐力训练影响,且不存在快、慢肌的差异。有助于我们对耐力训练工作肌IMTG供能和运动性骨骼肌适应的深入理解,更好地为科学进行耐力训练服务。
OBJECTIVE: To investigate the effects of different load-increasing endurance training methods on ATGL, HSL, CGI-58 and Perilipin gene expression in rats. Methods: Endurance training through the endurance training to build low-load endurance training, endurance load training and high load three kinds of training methods. The rats were sacrificed the next day after 4 weeks of endurance training. Gastrocnemius muscle and soleus muscle ATGL, HSL, CGI-58 and Perilipin gene expression were determined by reverse transcription polymerase chain reaction. The results showed that there was a difference in the expression of ATGLmR-NA in the gastrocnemius and soleus muscle of rats with increasing intensity endurance training. At moderate load increasing endurance training, the expression of ATGL mRNA in gastrocnemius muscle and soleus muscle peaked at the same time (P <0.05). There was no significant difference in ATGL mRNA expression between gastrocnemius and soleus in the same training mode (P> 0.05). However, the increment of endurance training of HSLmRNA reached its peak value with no significant difference (P> 0.05), but there was no difference between gastrocnemius and soleus (P> 0.05). Gastrocnemius muscle and soleus muscle in the same training mode, the maximum load training gastrocnemius CGI-58mRNA expression was significantly higher than the soleus muscle (P <0.05). There was no difference in the expression of per-ilipin mRNA between different intensities of endurance training and different muscles (P> 0.05). CONCLUSIONS: The expression of ATGL mRNA is related to the strength of endurance training and the fast and slow muscle differences of CGI-58 mRNA expression are only found during high-stress endurance training. However, the expression of HSL mRNA and Perilipin mRNA are not affected by endurance training and do not exist fast , Slow muscle differences. It helps us gain a deeper understanding of endurance training work muscle IMTG energization and exercise-induced skeletal muscle adaptation and better endurance training for science.