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目的研究广西地区门诊成人腹泻病例中星状病毒的感染状况和型别分布。方法2007年2月至2008年3月期间,收集广西南宁市某医院门诊成人腹泻患者粪便标本346份,应用RT-半巢式PCR检测星状病毒,对阳性标本进行测序验证和型别鉴定。结果在检测的346份粪便标本中,RT-PCR检出6份阳性,经测序证实均为星状病毒,检出率为1.73%(6/346)。病例全部集中在冬春季;发病年龄以17~29岁为主。6株毒株中有4株为HAstV-4型,占66.7%,另2株为HAstV-1型,占33.3%。结论首次证实广西地区成人腹泻病例中存在星状病毒感染,流行型别主要是HAstV-4和HAstV-1型。
Objective To study the infection status and type distribution of astrovirus in outpatients with adult diarrhea in Guangxi province. Methods From February 2007 to March 2008, 346 outpatients with adult diarrhea were collected from a hospital in Nanning, Guangxi. RT-PCR was used to detect astrovirus. The positive samples were verified by sequencing and type identification. Results Among the 346 stool samples tested, 6 were positive by RT-PCR and were all confirmed as astrovirus by sequencing. The detection rate was 1.73% (6/346). All cases concentrated in winter and spring; onset age of 17 to 29-year-old. Of the 6 strains, 4 strains were type HAstV-4, accounting for 66.7%. The other two strains were HAstV-1 type, accounting for 33.3%. Conclusion The first confirmed cases of adult diarrhea in Guangxi cases of astrovirus infection, the prevalence types are mainly HAstV-4 and HAstV-1 type.