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目的和方法 :本工作用红藻氨酸 (KA)癫痫模型 ,对用蝎毒处理该模型后海马内胆囊收缩素原mRNA(PCCKmRNA)表达进行原位杂交观察 ,并对国产东亚钳蝎粗毒抗癫痫反复发作的机制做了初步探讨。结果 :原位杂交的实验显示 ,三周KA后 ,实验对照组与空白对照组相比 ,腹侧海马尤其是海马门区PCCKmRNA阳性神经元数目明显减少 (P <0 .0 5 ) ;实验给药组大鼠 8例 ,其中有 6例腹侧海马门区PCCKmRNA阳性神经元数目有明显增加 ,显著高于实验对照组 (P <0 .0 1)和空白对照组 (P <0 .0 1)。结论 :蝎毒能选择性地增加癫痫敏感大鼠腹侧海马PCCKmRNA的表达。提示此效应很可能是蝎毒抗癫痫反复发作的重要的机制之一。
PURPOSE AND METHODS: In this study, kainic acid (KA) epilepsy model was used to observe the expression of cholecystokinin mRNA (PCCK mRNA) in the hippocampus after treating the model with scorpion venom. Anti-epileptic recurrent mechanism made a preliminary discussion. Results: In situ hybridization showed that the numbers of PCCK mRNA positive neurons in ventral hippocampus and hippocampus were significantly decreased in experimental control group compared with blank control group after three weeks of KA (P <0.05). Experiment The number of PCCK mRNA positive neurons in 6 cases of ventral hippocampus was significantly increased in 8 rats in the drug group, which was significantly higher than that in the experimental control group (P <0.01) and the blank control group (P <0.01) ). Conclusion: Scorpion venom can selectively increase the expression of PCCK mRNA in the ventral hippocampus of epileptic rats. Suggesting that this effect is likely to be one of the important mechanisms of recurrent seizures in the scorpion venom.