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用ＨＲＰ逆行追踪技术和体视学方法，探讨了胰岛素对移植在缺损运动神经元的脊髓内的胚胎神经元存活与生长有无促进作用。将１３～１４ｄ胚龄大鼠脊髓腹侧组织块，移植到受体大鼠左侧腰段缺损运动神经元的脊髓背外侧部。同时将受体鼠右侧带神经的 长伸肌移在左侧腰段脊椎旁，使其神经断端插入脊髓移植物的同一位置内。胰岛素组的胚胎移植物及覆盖其表面的硝酸纤维素膜，均经胰岛素生理盐溶液浸泡过，对照组仅用单纯生理盐溶液浸泡。术后大鼠存活６周。在移植的肌肉内注入ＨＲＰ作逆行追踪。结果：１．胰岛素组胚胎移植物的体积为０．４２５２±０．０７３６ｍｍ３；对照组的为０．１３８３±０．０２９６ｍｍ３。２．胰岛素组移植物神经元胞体体积为０．０１７２±０．００４１ｍｍ３；对照组的为０．００３０±０．００１１ｍｍ３。３．胰岛素组移植物神经元数目为５５１８±１３４３个；对照组的为１２１４±４３７个。４．胰岛素组移植物内和移植物邻近的受体脊髓组织中，可见ＨＲＰ阳性神经元，而对照组则未观察到。以上结果提示：胰岛素能促进移植的胚胎脊髓神经元的存活与生长；有些神经元还支配了移植的肌肉，其中一些神经元很可能迁移到了受体脊髓组织中。
HRP retrograde tracing technique and stereological methods were used to explore the effect of insulin on the survival and growth of embryonic neurons transplanted in the spinal cord of deficient motor neurons. The ventral tissue of rats spinal cord from 13 to 14 days old were transplanted to the dorsolateral part of the spinal cord in the left lumbar defect motor neurons of the recipient rats. At the same time, the right extensor nerve long extensor of the recipient mouse is moved to the left lumbar vertebra, and the nerve ends are inserted into the same position of the spinal cord implants. Insulin group of embryo grafts and covering the surface of the nitrocellulose membrane, were soaked in insulin physiological saline solution, the control group was immersed in saline solution only. Postoperative rats survived for 6 weeks. HRP was injected into the transplanted muscle for retrograde tracing. Results: 1. The volume of the insulin group embryo graft was 0.4252 ± 0.0736 mm3 and that of the control group was 0.1383 ± 0.0296 mm3.2. Insulin group transplantation neuronal cell volume was 0.0172 ± 0.0041mm3; control group was 0.0030 ± 0.0011mm3.3. The number of neurons in the insulin group was 5518 ± 1343 and in the control group 1214 ± 437. 4. HRP-positive neurons were seen in the recipient spinal cord tissue in and near the graft of insulin group, but not in the control group. The above results suggest that: Insulin can promote the survival and growth of transplanted embryonic spinal cord neurons; some neurons also dominate the transplanted muscle, some of which are likely to migrate to the recipient spinal cord tissue.