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方法：用氯化钾诱发ＳＤ大鼠皮层扩散性抑制（ＣＳＤ），改良 Ｌｏｎｇａ法制作大鼠 ＭＣＡＯ模型，用地高辛标记的 ｃＲＮＡ探针，行原位杂交检测谷氨酸载体ＥＡＡＣ１ ｍＲＮＡ表达。结果：假手术组脑片上单位面积ＯＤ为（２ １８９±２６３），阳性颗粒数为（４３．２±４．７）个。缺血 ３０ｍｉｎ时皮层ＥＡＡＣ１ ，ｍＲＮＡ表达明显上调，经ＣＳＤ处理后再缺血时，与对照组比较表达水平均明显增高，其中Ｌｉ ３０ｍｉｎ时最高。结论：ＣＳＤ的作用可能与诱导ＥＡＡＣ１合成，增加谷氨酸的摄取有关。
Methods: CSD was induced by potassium chloride (CSD). MCAO model was established by modified Longa method. The glutamate transporter EAAC1 mRNA was detected by in situ hybridization with digoxigenin-labeled cRNA probe. Results: In the sham operation group, the unit area OD of the brain slices was (2 189 ± 263) and the number of positive particles was (43.2 ± 4.7). The expression of EAAC1 and mRNA in the cortex was significantly increased at 30min after ischemia, and the expression of EAAC1 and mRNA was significantly increased in the ischemic group after CSD treatment. Conclusion: The effect of CSD may be related to induction of EAAC1 synthesis and increase of glutamate uptake.