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已知人类免疫缺陷症病毒(HIV)能通过输注全血和血浆制品(特别是因子Ⅷ或Ⅸ浓制剂传播,通过加热或紫外线处理可灭活HIV。最近有人报道,从经免疫球蛋白(Ig)治疗后的病人中分离到HIV逆转录病毒。所以对目前的血液制品生产过程是否足以灭活HIV作出评价就显得很重要。本实验采用来源于1例成人T细胞白血病患者的新细胞系SKT-1B。作者将处于生长期的靶细胞,用2μg/ml的溴化已二甲铵在室温下处理30分钟后,将沉淀的50万个细胞重悬于0.2ml的HIV溶液中,置室温45分钟后加2
It is known that human immunodeficiency virus (HIV) can inactivate HIV by infusion of whole blood and plasma products, particularly factor VIII or IX concentrates, by heat or UV treatment. It has recently been reported that immunization with immunoglobulins Ig), so it is important to evaluate whether the current production of blood products is enough to inactivate HIV.This experiment used a new cell line derived from one patient with adult T-cell leukemia SKT-1B. The target cells in the growth phase were treated with 2μg / ml of dimethyl ammonium bromide at room temperature for 30 minutes. After the 500,000 cells were resuspended in 0.2ml of HIV solution, Add 45 after 45 minutes at room temperature