实验用健康成年雄性大鼠21只,体重250克左右,随机分为正常对照组(n=10),创伤性休克组(n=11),实验大鼠在乙醚轻麻下,用直径1.5毫米线绳;腹侧经腹股沟;背侧经大转子上缘;用弹簧拉力计以相当5kg之力结扎双侧大鼠后肢二小时半,形成创伤性休克模型。断头处死动物,取腰段脊髓,进行P物质放射免疫测定和免疫细胞学观察,采用间接免疫荧光法,脊髓用0.4％对位苯醌固定,恒冷籍切 Twenty-one healthy adult male rats weighing 250 g were randomly divided into normal control group (n = 10) and traumatic shock group (n = 11). The rats were anesthetized with diethyl ether Line; the ventral groin; dorsal to the upper edge of the greater trochanter; with a spring tension meter with a force of 5kg ligation of bilateral hind legs two and a half hours to form a traumatic shock model. The animals were decapitated and the lumbar spinal cord was taken for radioimmunoassay and immunocytology. The indirect immunofluorescence method was used to determine the spinal cord injury. The spinal cord was fixed with 0.4% para-benzoquinone,