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目的探讨冠通方对大鼠心肌梗死后炎症细胞因子c反应蛋白(CRP)、白细胞介素1β(IL-1β)的mRNA表达的影响。方法成功造模的急性心肌梗死SD大鼠30只随机分为冠通方组、安慰剂组、模型组,每组10只。另设假手术组,空白对照组,每组10只。冠通方组冠通方灌胃,安慰剂组生理盐水灌胃,其余不做特殊处理。二月后处死大鼠,取心肌组织,用rt-qPCR法测定CRP、IL-1β的mRNA表达结果。结果 CRP mRNA表达,模型组及安慰剂组比较表达量较空白对照组明显升高(P<0.01),假手术组较空白组未见明显升高(1.15±0.11,P>0.05);冠通方组较安慰剂组表达量降低,有显著差异(P<0.01);安慰剂组较模型组未见明显差异(P>0.05)。IL-1βmRNA表达,模型组及安慰剂组比较表达量较空白对照组明显升高(P<0.01);假手术组较空白组未见明显升高(1.11±0.48,P>0.05);冠通方组较安慰剂组表达量降低,有显著差异(P<0.01);安慰剂组较模型组未见明显差异(P>0.05)。结论 CRP、IL-1β参与了急性心梗后炎症反应,中药冠通方对这两种炎症因子有一定抑制作用。
Objective To investigate the effects of Guantong Formula on mRNA expression of inflammatory cytokines c-reactive protein (CRP) and interleukin-1β (IL-1β) after myocardial infarction in rats. Methods Thirty SD rats with acute myocardial infarction were randomly divided into Guan Tong Fang group, placebo group and model group, with 10 rats in each group. Another set of sham operation group, blank control group, 10 in each group. Guan Tong Fang Guan Tong Fang prescription gavage, placebo saline infusion, the rest do not do special treatment. Rats were sacrificed after February, myocardial tissue was taken and mRNA expression of CRP and IL-1β was measured by rt-qPCR. Results The expression of CRP mRNA in model group and placebo group was significantly higher than that in blank control group (P <0.01), but not in sham operation group (1.15 ± 0.11, P> 0.05) Compared with the placebo group, the placebo group showed a significant decrease (P <0.01), and no significant difference between the placebo group and the model group (P> 0.05). Compared with blank control group, the expression of IL-1βmRNA in model group and placebo group was significantly higher than that in blank control group (P <0.01); sham-operated group had no significant increase (1.11 ± 0.48, P> 0.05) Compared with the placebo group, the placebo group showed a significant decrease (P <0.01), and no significant difference between the placebo group and the model group (P> 0.05). Conclusion CRP and IL-1β are involved in the inflammatory reaction after acute myocardial infarction. Guanotong Decoction has certain inhibitory effect on these two inflammatory factors.