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目的：研究在缺氧缺糖状态下，大鼠大脑皮层神经元膜电压依赖性钾通道的开关动力学变化。方法：急性分离大鼠大脑皮层神经元和细胞贴附膜片钳技术。结果：与对照组相比，在缺氧缺糖状态下，电压依赖性钾通道开放时间常数τ１和τ２分别由对照组的０．４２９ｍｓ和８．２０９ｍｓ增加至１．８５５ｍｓ和１７．４６４ｍｓ（Ｐ＜０．０１），关闭时间常数τ和通道的电流幅度无明显变化。结论：缺氧缺糖可导致大鼠皮层神经元电压依赖性钾通道开放显著增加，对胞外钾离子聚集起重要作用，从而提高细胞的兴奋性，参与神经元的损伤。
OBJECTIVE: To study the switching dynamics of voltage-dependent potassium channels in rat cerebral cortical neurons under hypoxia-deprivation conditions. Methods: Acutely isolated rat cerebral cortical neurons and cell patch clamp technique. Results: Compared with the control group, the open-circuit time constants τ1 and τ2 of voltage-dependent potassium channels were increased from 0.429ms and 8.209ms in the control group to 1.855ms and 17.464ms (P <0.01), the closing time constant τ and the current amplitude of the channel did not change significantly. Conclusion: Oxygen-glucose-deprivation can lead to the significant increase of voltage-dependent potassium channels in rat cortical neurons and play an important role in the accumulation of extracellular potassium ions, thereby increasing the excitability of cells and participating in the neuronal damage.