论文部分内容阅读
目的 :研究乙型肝炎病毒 (HBV)前C区热点变异对宿主细胞HLA Ⅰ分子表达的影响 .方法 :构建HBV前C区野毒株及A83,A83/A86变异株真核表达质粒 ,转染HepG2细胞 ,鉴定目的基因在宿主细胞中的生物活性 ,流式细胞术检测HLA Ⅰ分子的表达 .结果 :PCR和ELISA法分析能检测到目的DNA片段
Objective: To study the effect of HBV pre-C mutation on the expression of host HLA ¢ ô molecules.Methods: The eukaryotic expression plasmids of the pre-HBV C-strain and A83, A83 / A86 strains were constructed and transfected HepG2 cells to identify the biological activity of the target gene in host cells and the expression of HLA class I molecules by flow cytometry.Results PCR and ELISA analysis could detect the target DNA fragment