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植物中糖的种类和含糖量的分析对于选种和控制人工培育条件有着重要的意义,为研究红松幼树生产过程中糖的代谢途径,对样品来源困难、含量差异小的样品,采用原子吸收法间接测定红松幼树不同部位还原糖的含量和总糖含量。 鉴于碱性铜络离子能被特球的醛——葡萄糖还原成Cu_2O这一特性。本实验采用斐林试剂法使还原糖在碱性溶液中将Cu~(++)还原成一价的Cu_2O后,收集Cu_2O沉淀,溶于硝酸后,在波长3247mm处用原子吸收分光光度计测定。还原糖的含量在0.5—5μg/ml范围内呈良好的绒性关系。样品含量在0.05mg/g以上即可测出。本法适用于样品含量少,样品间差异小的生物分析。测定的变异系数为1.4％,回收率92.8％。
The analysis of the type and sugar content of sugars in plants is of great significance for the selection and control of artificial cultivation conditions. To study the metabolic pathways of sugar during the production of young trees of Pinus koraiensis, the samples with difficult sample sources and small differences in content were used. Atomic Absorption Method for Indirect Determination of Reducing Sugar Content and Total Sugar Content in Different Parts of Young Trees of Pinus koraiensis In view of the characteristics of the alkaline copper ions can be reduced to Cu 2 O by the aldehyde of the special sphere. In this experiment, the reduction of Cu~(++) to monovalent Cu2O was carried out using reducing agent sugar in an alkaline solution. The Cu2O precipitate was collected, dissolved in nitric acid, and measured at an atomic absorption spectrophotometer at a wavelength of 3247 mm. The content of reducing sugar showed a good velvety relationship in the range of 0.5-5 μg/ml. Samples above 0.05mg/g can be measured. This method is applicable to biological analysis with small sample content and small difference between samples. The coefficient of variation measured was 1.4% and the recovery was 92.8%.