论文部分内容阅读
目的:探讨小白菊内酯(Par)对涎腺腺样囊性癌细胞系SACC-83的增殖抑制作用,并阐明其相关作用机制。方法:不同浓度(5.0、7.5、15.0、30.0、40.0和80.0mg·L-1)Par作用于体外培养的SACC-83细胞系,同时设空白对照组;MTT比色法观察Par对SACC-83细胞系的生长抑制情况;流式细胞仪观察Annexin V-FITC/PI双染后细胞凋亡状况;Western blotting法检测Livin蛋白的表达情况。结果:不同浓度Par组SACC-83细胞生长受到明显的抑制,与空白对照组比较增殖抑制率明显增加,呈浓度和时间依赖性;经7.5、15.0和30.0mg·L-1 Par作用24h后,各组细胞凋亡率分别为14.7%±2.1%、30.5%±3.4%和38.7%±2.7%,与空白对照组凋亡率(3.2%±0.9%)比较,差异具有统计学意义(P<0.01);不同浓度Par组Livin蛋白的表达明显下调,与空白对照组比较差异有统计学意义(P<0.05),且各浓度组之间比较差异有统计学意义(P<0.01)。结论:Par对SACC-83细胞系有显著的增殖抑制作用,Livin蛋白的表达可能与其作用机制有关。
Objective: To investigate the inhibitory effect of parthenolide (SAL) on the proliferation of human salivary adenoid cystic carcinoma cell line SACC-83 and to elucidate its mechanism of action. Methods: SACC-83 cell lines were treated with various concentrations of Par (5.0, 7.5, 15.0, 30.0, 40.0 and 80.0 mg · L -1) and blank control group. MTT assay was used to observe the effect of Par on SACC-83 Cell growth inhibition; Flow cytometry Annexin V-FITC / PI double staining of apoptosis status; Western blotting assay Livin protein expression. Results: The growth of SACC-83 cells was significantly inhibited in different concentrations of Par group, and the proliferation inhibition rate was significantly increased in a concentration-and time-dependent manner compared with the blank control group. After treated with 7.5, 15.0 and 30.0 mg · L-1 Par for 24 h, The apoptosis rate of each group was 14.7% ± 2.1%, 30.5% ± 3.4% and 38.7% ± 2.7%, respectively, which was significantly different from that of the blank control group (3.2% ± 0.9%) (P < 0.01). The expression of Livin protein was significantly down-regulated in different concentrations of Par group compared with the blank control group (P <0.05), and there was significant difference between each concentration group (P <0.01). Conclusion: Par has a significant inhibitory effect on SACC-83 cell line, and the expression of Livin may be related to its mechanism of action.