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本文作者建立了一种简单、快速、效果较理想的分离肝脏淋巴细胞的方法。以往分离肝脏淋巴细胞的唯一方法是通过有限稀释法克隆T 细胞,IL-2维持生长。这种方法选择性诱生了某些细胞,故难以反映原位肝组织的情况。作者改进肿瘤浸润淋巴细胞(TIL)的分离方法,用于肝脏淋巴细胞的分离。这种方法包括以下几步:机械性分离;切碎肝组织;酶消化切碎的组织;连续2次Ficoll-Hypaqne 密度梯度离心。第一
The author of the paper established a simple, rapid and effective method of separating liver lymphocytes. In the past, the only way to isolate liver lymphocytes was to clone T cells by limiting dilution, and IL-2 maintained its growth. This method selectively induced some cells, it is difficult to reflect the situation of liver tissue in situ. The authors improve the method for the separation of tumor-infiltrating lymphocytes (TILs) for the isolation of liver lymphocytes. This method consists of the following steps: mechanical separation; shredding of liver tissue; enzymatic digestion of shredded tissue; and continuous Ficoll-Hypaqne density gradient centrifugation twice. the first