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高效疏水色谱(HPHIC)用于分离分子量高达1000 000的IgM类单克隆抗体还未见文献报道。我们用HPHIC对杂交瘤培养上清中的IgM类单克隆抗体进行了分离纯化,现报告如下。
High-performance hydrophobic chromatography (HPHIC) for the isolation of IgM monoclonal antibodies with molecular weights up to 1000 000 has not been reported in the literature. We use HPHIC hybridoma culture supernatant IgM monoclonal antibody was isolated and purified, are reported as follows.