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用化学发光法测定：人血ＰＭＮ细胞受ＰＭＡ刺激发生呼吸暴发产生的活性氧；黄嘌呤－黄嘌呤氧化酶体系产生的Ｏ；Ｖｉｔｃ－ＣＵ２＋－酵母多糖产生的·ＯＨ及Ｈ２Ｏ２的释放，同时也观察了维拉帕米对上述体系产生的自由基的影响。结果提示，维拉帕米对ＰＭＮ细胞的呼吸暴发有显著的抑制作用；对Ｏ、Ｈ２Ｏ２有清除作用，呈剂量依赖性，其ＩＣ５０分别为２８．１，２２．３ｎｍｏｌ·Ｌ－１；对·ＯＨ则无作用。
Chemiluminescence method was used to determine the release of · OH and H2O2 produced by Oxygen produced by xanthine-xanthine oxidase system and by Vitc-CU2 + -Zymopolysaccharide in human PMN cells stimulated by PMA. The effects of verapamil on the free radicals produced by the above system were observed. The results suggest that verapamil has a significant inhibitory effect on respiratory outbreak in PMN cells; it has a scavenging effect on O and H2O2 in a dose-dependent manner with IC50 of 28.1 and 22.3 nmol·L-1, respectively; OH is no effect.