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目的 研究地塞米松 (dexamethasone)、重组人碱性成纤维细胞生长因子 (recombinanthumanbasicfibroblastgrowthfator,rhFGF)、重组人骨形态发生蛋白 -2 (recombinanthumanbonemorpho geneticprotein -2 ,rhBMP -2 )三种骨生长相关因子对兔骨膜细胞生长及分化的量效作用 ,为其在骨组织工程中的应用提供实验基础。 方法 体外分离培养兔骨膜细胞 ,分别与终浓度为 10 - 8,10 - 7,10 - 6 mol/L的地塞米松 ;终浓度为 50 ,2 0 0 ,50 0ng/ml的rhFGF ;终浓度为 50 ,50 0 ,10 0 0ng/ml的rhBMP -2共培养。分别于 4,7d后终止培养并测定细胞生长及分化指标。 结果 10 - 6 mol/L地塞米松显著抑制细胞蛋白合成 ,且对骨膜细胞碱性磷酸酶 (alkalinephosphatase ,ALP)表达无明显影响。各浓度组rhFGF显著促进了细胞蛋白合成量 ,而表现出明显的ALP活性抑制。各浓度组rhBMP -2对细胞增殖无明显影响 ;50ng/ml的rhBMP -2不影响骨膜细胞的ALP活性 ;当浓度增加到 50 0ng/ml与 10 0 0ng/ml时 ,ALP活性显著增加 (P <0 .0 1)。 结论 rhBMP -2在不影响骨膜细胞增殖的前提下 ,适当的浓度能够显著促进骨膜细胞向成骨细胞转化 ,在骨组织工程的研究中有重要的应用价值
Objective To investigate the effects of dexamethasone, rhBGF and rhBMP-2 on the periosteum Cell growth and differentiation of the quantitative effect for its application in bone tissue engineering provide experimental basis. Methods Rabbit periosteal cells were isolated and cultured in vitro, respectively, with dexamethasone at a final concentration of 10 - 8, 10 - 7 and 10 - 6 mol / L; rhFGF at a final concentration of 50, 200, 500 ng / ml; 50, 50 0, 100 ng / ml of rhBMP-2 were co-cultured. After 4 and 7 days, the culture was terminated and the cell growth and differentiation indexes were determined. Results 10 - 6 mol / L dexamethasone significantly inhibited cellular protein synthesis and had no significant effect on alkaline phosphatase (ALP) expression in periosteal cells. Each concentration of rhFGF significantly increased the amount of cellular protein synthesis, and showed a significant inhibition of ALP activity. The concentration of rhBMP-2 had no significant effect on the cell proliferation; rhBMP-2 of 50ng / ml did not affect the ALP activity of periosteal cells; ALP activity increased significantly when the concentration was increased to 50 0ng / ml and 100ng / ml <0 .0 1). Conclusion rhBMP-2 can significantly promote the transformation of periosteal cells into osteoblasts under the premise of not affecting the proliferation of periosteal cells, and it has an important value in the study of bone tissue engineering