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AIM:To investigate the in vitro anti-hepatocellular carcinoma(HCC)activity of docetaxel against SMMC-7721 HCC cellsand its possible mechanism.METHODS:The HCC cells were given different concentrationsof docetaxel and their growth was measured by colonyforming assay.Cell cycle and apoptosis were analyzed byflow cytometry and fluorescence microscopy(acridineorange/ethidium bromide double staining,AO/EB),as wellas electronic microscopy.The SMMC-7721 HCC cell reactiveoxygen species(ROS)and glutathione(GSH)were measuredafter given docetaxel.RESULTS:Docetaxel inhibited the hepatocellular carcinomacells growth in a concentration dependent manner withIC_(50)5×10~(-10)M.Marked cell apoptosis and G2/M phase arrestwere observed after treatment with docetaxel ≥10~(-8)M.Docetaxel promoted SMMC-7721 HCC cells ROS generationand GSH deletion.CONCLUSION:Docetaxel suppressed the growth of SMMC-7721 HCC cells in vitro by causing apoptosis and G2/M phasearrest of the human hepatoma cells,and ROS and GSH mayplay a key role in the inhibition of growth and induction ofapoptosis.
AIM: To investigate the in vitro anti-hepatocellular carcinoma (HCC) activity of docetaxel against SMMC-7721 HCC cells and its possible mechanism. METHODS: The HCC cells were different given concentrations of docetaxel and their growth was measured by colonyforming assay. Cell cycle and apoptosis were analyzed by flow cytometry and fluorescence microscopy (acridineorange / ethidium bromide double staining, AO / EB), as wellas electronic microscopy.The SMMC-7721 HCC cell reactiveoxygen species (ROS) and glutathione (GSH) were measuredafter given docetaxel.RESULTS: Docetaxel bromide The hepatocellular carcinoma cells growth in a concentration dependent dependent manner with IC 50 (50) 5 × 10 -10 M.Marked cell apoptosis and G2 / M phase arrestwere observed after treatment with docetaxel ≥10 -8 M. Docetaxel promoted SMMC- 7721 HCC cells ROS generation and GSH deletion. CONCLUSION: Docetaxel suppressed the growth of SMMC-7721 HCC cells in vitro by caused apoptosis and G2 / M phasearrest of the human hepatoma cells, and ROS and GSH mayplay a key role in the inhibition of growth and induction ofapoptosis.