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为了开拓抗虫基因源,培育抗性稳定的水稻抗虫品种,采用感虫的栽培稻雄性不育株为母本,以高抗褐稻虱的野生稻为父本进行有性杂交。通过用改良的苗期群体筛选法,对其后代进行抗性鉴定,证实其抗性可以遗传,已成功地获得了抗褐稻虱的杂种后代。并根据飞虱在抗虫植株上的生存率、群体建立、蜜露量和稻株的受害级别等指标与感虫对照种TN1上的差异显著性作比较,综合评定杂种后代对褐稻虱的抗性机制。结果表明,测试的N482(A)/101392组合的F_5、F_6、F_7和H_4等几个后代对褐稻虱的抗性机制均为抗生性。
In order to develop the insect-resistant gene source, cultivate the resistance-resistant rice insect-resistant varieties, the male sterile plants of the susceptible cultivated rice were used as the female parent and the wild rice resistant to the brown planthopper as the male parent for the sexual hybrids. Through the improved seedling population screening method, the resistance of their offspring was identified, which proved that the resistance can be inherited, and the offspring of the brown planthopper resistance have been successfully obtained. Based on the differences in the survival rates of planthoppers in insect-resistant plants, population establishment, honeydew amount and the damage level of rice plants compared with the susceptible TN1, the comprehensive evaluation of the offspring of hybrids on the brown planthopper Resistance mechanism. The results showed that the resistance mechanisms of several progeny of F48, F_6, F_7 and H_4 of N482 (A) / 101392 tested to the brown planthopper were all antibiotic.