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目的探讨兔脑局部冷冻对兔全身微循环的影响及蝮蛇抗栓酶对其的干预作用。方法单纯冷冻组、冷冻十生理盐水组、冷冻＋蝮蛇抗栓酶组各８例，分别检测脑局部冷冻前后不同时相（Ｔ０、ＴＯ．５、Ｔ１、Ｔ２、Ｔ３等）动物的球结膜微循环流态、形态、红细胞聚集及管周状态的变化，对检测结果进行统计学分析。结果单纯冷冻组、冷冻＋生理盐水组脑局部冷冻后球结膜微循环血管密度减少，微动脉变细，血流速度减慢，血液流态呈粒流或粒缓流，红细胞中度聚集，可见大小不等的白微栓流动，血细胞贴壁、翻滚。两组冷冻后不同时相（Ｔ０．５、Ｔ１、Ｔ２、Ｔ３）分别与冷冻前（Ｔ０）自身比较，差别有显著性（Ｐ＜０．０５，Ｐ＜０．０１）。冷冻＋蝮蛇抗栓酶组球结膜微循环状态积分，脑局部冷冻前后比较，差别无显著性（Ｐ＞０．０５）。冷冻＋蝮蛇抗栓酶组冷冻后不同时相（Ｔ０．５、Ｔ１、Ｔ２、Ｔ３）微循环状态积分明显优于单纯冷冻组和冷冻＋生理盐水组，组间比较差别有显著性（Ｐ＜０．０５）。单纯冷冻组与冷冻＋生理盐水组脑局部冷冻后，球结膜微循环状态积分，组间比较差别无显著性（Ｐ＞０．０５）、结论兔脑局部冷冻能引起全身微循环功能障碍，其机理可能是；兔脑局部冷冻造成脑组织局部微循环障碍形成?
Objective To investigate the effect of local freezing of rabbit brain on systemic microcirculation and the intervention of viper antithrombin. Methods Eight cases of frozen-thawed group, frozen-saline group and frozen-viper antithrombin group were used to detect the conjunctiva of the animals at different phases (T0, TO.5, T1, T2, T3, Microcirculation flow pattern, morphology, erythrocyte aggregation and changes in the status of the tube circumference, the test results were statistically analyzed. Results In the group of cryopreservation alone and in the group of cryo + saline, the density of bulbar conjunctiva microcirculation decreased, the arterioles became thinner and the blood flow velocity slowed down. The blood flow was in the form of granular flow or granular flow, and the red blood cells were moderately aggregated. White micro-bolt of varying sizes flow, blood cells adherent, rolling. There were significant differences between the two groups (T0.5, T1, T2 and T3) before freezing (T0) after freezing (P <0.05, P <0.01). Frozen + viper antithrombotic group of conjunctival microcirculation state points, brain freezing before and after local comparison, the difference was not significant (P> 0.05). The integral of microcirculation in frozen + viper antithrombin group after freezing at different phases (T0.5, T1, T2, T3) was significantly better than that of freezing group and freezing + saline group (P <0.05). After cryoablation and freezing + saline group, the cerebral conjunctival microcirculation state integral was not significantly different between the two groups (P> 0.05). Conclusion Local freezing of rabbit brain can cause systemic microcirculation dysfunction. Mechanism may be; local brain tissue caused by local freezing of microcirculation of the brain formation?