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发病机理近年来通过实验室的研究,对百日咳的发病机理有了进一步的了解。 (一)百日咳杆菌对纤毛上皮细胞的损害Standfast用百日咳杆菌毒素对新鲜羊肺制备的纤毛细胞作用的研究,证明百日咳杆菌的毒素可以使纤毛上皮细胞的纤毛完全麻痹。Hoperwell等应用低毒力的百日咳杆菌给鼠作颅内感染,结果发现百日咳杆菌粘附于室管膜的纤毛上皮细胞上。以后Collier应用田鼠的气管作器官培养,以研究百日咳杆菌对呼吸道上皮细胞的作用,结果发现被百日咳杆菌感染24小时的气管,在纤毛细胞的纤毛间有许多百日咳杆菌。48小时后纤毛细胞形态上有明显的变化,包括细胞浆空泡形成及核的破裂溶解,电镜检查百日咳杆菌仍在细胞外,没有证明侵入细胞内。72小时后仅非纤毛细胞残存,它既没有细菌附着,也没有形态学的改变,电镜检查超微结构正常。他们还应用~(14)C标记的氨基酸检查
Pathogenesis In recent years through laboratory research, the pathogenesis of whooping cough has a better understanding. (A) Bordetella pertussis damage to the ciliated epithelial cells Standfast with Bordetella pertussis to fresh goat lung preparation of ciliary cells, to prove that toxin B pertussis to make ciliated epithelial cells completely paralyzed cilia. Hoperwell and other applications of low-virulent Bordetella pertussis intracranial infection in mice, the results found that B. pertussis adhesion to the ependymal ciliated epithelial cells. Later Collier application of voles tracheal organ culture to study the role of Bordetella pertussis on the respiratory epithelial cells and found that the infection of the trachea by pertussis bacteria for 24 hours in the cilia of the cilia there are many Bordetella pertussis. After 48 hours, there were obvious changes in the morphology of ciliated cells, including the formation of cytoplasmic vacuoles and the rupture and lysis of nuclei. The results of electron microscopy showed that B. pertussis was still outside the cells and did not show invasion into cells. After 72 hours, only the non-ciliated cells remained. They had neither bacterial attachment nor morphological changes and their ultrastructures were normal by electron microscopy. They also applied ~ (14) C-labeled amino acid tests