论文部分内容阅读
以Ｇｅｎｅｌａｂｓ的ＨＥＶＩｇＧＥＬＩＳＡ试剂盒和抗人μＦ（ａｂ’）＿２—ＨＲＰ检测抗ＨＥＶＩｇＧ和ＩｇＭ，从上海地区４６７例急性病毒性肝炎病人和８４例健康人血清中分别检出抗ＨＥＶＩｇＧ阳性１０５例（２２％）和１１例（１３％）（Ｐ＞０．０５）。从上述病人共检出抗ＨＥＶＩｇＭ阳性６４例（１４％），其中３６例抗ＨＥＶＩｇＭ阳性出自１１１例急性非甲非乙非丙型肝炎，２８例抗ＨＥＶＩｇＭ阳性出自３３３例急性甲型肝炎病人，而２３例急性乙型和丙型肝炎病人，８４例健康人以及另外的２４例非病毒性肝炎（内４例类风湿因子阳性）病人血清均为抗ＨＥＶｌｇＭ阴性。结果表明：在检测抗ＨＥＶＩｇＭ的ＥＬＩＳＡ系统中，应用抗人μＦ（ａｂ’）＿２—ＨＲＰ作为酶标第二抗体，取得了满意的结果；上海地区急性非甲非乙非丙型肝炎中，３２％为戊型肝炎，急性甲型肝炎病人中８％同时感染了ＨＡＶ和ＨＢＶ。
HEV IgGG and IgM were detected by Genelabs HEVIgG ELISA kit and anti-human μF (ab ’) _2-HRP, and 105 positive anti-HEVIgG antibodies were detected in 467 cases of acute viral hepatitis and 84 healthy human serum in Shanghai %) And 11 cases (13%) (P> 0.05). A total of 64 anti-HEVIgM positives (14%) were detected from the above patients, of which 36 were anti-HEVIgM positive from 111 non-A non-B non-C hepatitis and 28 anti-HEVIgM positive were from 333 A patients, Serum samples from 23 patients with acute hepatitis B and C, 84 healthy individuals, and another 24 patients with non-viral hepatitis (within 4 rheumatoid factor positive) were negative for HEV IgM. The results showed that satisfactory results were obtained by using anti-human μF (ab ’) _ 2-HRP as the second antibody in the ELISA system for detecting anti-HEVIgM. In Shanghai, acute non-A non-B hepatitis B % Of hepatitis E, 8% of patients with acute hepatitis A are infected with both HAV and HBV.