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研究PHA对日本血吸虫成虫培养细胞的促增殖作用。方法用浓度为333．3ug／ml的PHA处理日本血吸虫成虫培养细胞24h，在光镜和电镜下观察培养细胞的增殖情况。结果在光镜下发现在老化的培养细胞中间出现一些类似于淋巴母细胞样的新细胞；在光镜和扫描电镜下观察到有呈哑铃状的成对细胞；在透射电镜下观察到有细胞核膜消失，染色质变成粗颗粒状．中心粒一分为二，两中心粒之间还有纺锤丝牵引；还观察到处于分裂早期的细胞，核中染色质浓缩成四块状，核膜有部分消失、结论PHA对日本血吸虫成虫培养细胞有一定的促增殖作用。
Study of PHA on Schistosoma japonicum adult cultured cells to promote proliferation. Methods The cultured cells of adult Schistosoma japonicum were treated with PHA at the concentration of 333.3ug / ml for 24 hours. The proliferation of cultured cells was observed under light microscope and electron microscope. RESULTS: Under the light microscope, some new cells similar to lymphoblastoid cells appeared in the middle of aged cultured cells. Dumbbell-like cells were observed under light microscope and scanning electron microscope. The nuclei were observed under transmission electron microscope Membrane disappears, the chromatin becomes coarse granular. The centriole was divided into two parts, there was spindle traction between the two centrioles and the cells in the early stage of division were also observed. The chromatin in the nucleus was condensed into four lumps, and the nuclear membrane partially disappeared. Conclusions PHA cultured adult schistosomiasis japonica Cells have a certain role in promoting proliferation.