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用原位杂交法及激光光密度计灰度扫描半定量法，比较了老年组（２４月龄）与青年组（６周龄）Ｗｉｓｔａｒ大鼠离体培养的主动脉ＶＳＭＣ（５－１０代）ｃ－ｍｙｃ与ＨＳＰ７０基因的表达及红细胞抗高血压因子（ＡＨＦ）、去甲肾上腺素（ＮＥ）与人参皂甙对上述基因表达的影响。结果表明：１．增龄导致的ＶＳＭＣ增殖很可能与ｃ－ｍｙｃ基因的过度表达有关，ＮＥ、ＡＨＦ和人参皂甙可以通过调控ｃ－ｍｙｃ基因的表达来影响ＶＳＭＣ增殖。２．增龄伴随的应激能力下降可能与ＨＳＰ７０基因表达的减少有关。３．ＨＳＰ７０基因也可能参与了ＶＳＭＣ增殖与分化的调控，ＮＥ、ＡＨＦ与人参皂甙可能通过调控ＨＳＰ７０基因的表达来影响ＶＳＭＣ的增殖
In situ hybridization and laser densitometry were used to detect the aortic VSMC (5-10 passages) in Wistar rats aged 24 months and 6 weeks. c-myc and HSP70 gene expression and the effects of erythrocyte antihypertensive factor (AHF), norepinephrine (NE) and ginsenoside on the above gene expression. The result shows: 1. VSMC proliferation induced by aging is likely related to the overexpression of c-myc gene. NE, AHF and ginsenoside can affect VSMC proliferation by regulating the expression of c-myc gene. 2. Decreased stress associated with aging may be related to the decrease of HSP70 gene expression. 3. HSP70 gene may also be involved in the regulation of VSMC proliferation and differentiation, NE, AHF and ginsenosides may affect VSMC proliferation by regulating the expression of HSP70 gene