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以乙肝疫苗、人喉癌细胞膜抗原为抗原，猪脾细胞为效应细胞，经体外免疫后收集应答细胞，制备ＰＳＨＢＶ－ＴＦ和ＰＳＡＣ－ｉＲＮＡ。通过抗原特异性细胞免疫功能试验证实，ＰＳＨＢＶ－ＴＦ和ＰＳＡＣ－ｉＲＮＡ都能转移特异性细胞免疫功能。采用体外免疫法制备ＰＳＨＢＶ－ＴＦ和ＰＳＡＣ－ｉＲＮＡ是可行的，并且具有诸多优点。
Hepatitis B vaccine and human laryngeal cancer cell membrane antigen were used as antigen and porcine spleen cells as effector cells. Response cells were collected after in vitro immunization to prepare PSHBV-TF and PSAC-iRNA. Through antigen-specific cellular immune function tests confirmed that both PSHBV-TF and PSAC-iRNA can transfer specific cellular immune function. The preparation of PSHBV-TF and PSAC-iRNA by in vitro immunization is feasible and has many advantages.