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目的探讨对氨基水杨酸钠(PAS-Na)对铅致幼龄大鼠学习记忆损伤及海马氨基酸类神经递质改变的影响。方法 50只雌雄各半初断乳SD大鼠随机分为对照组,染铅组,低、中、高剂量PAS-Na治疗(L-、M-、H-PAS)组。染铅、L-、M-和H-PAS组大鼠腹腔注射(ip)15 mg/kg醋酸铅,对照组ip等容量生理盐水,1次/d,连续2周。然后L-、M-和H-PAS组背部皮下注射(sc)100、200和300 mg/kg PAS-Na,染铅组和对照组背部sc等容量生理盐水,1次/d,连续3周。用Morris水迷宫测定大鼠学习记忆能力,石墨炉原子吸收分光光度法测定大鼠血铅含量,高效液相色谱法测定海马谷氨酸(Glu)、谷氨酰胺(Gln)和γ-氨基丁酸(GABA)含量。结果与对照组相比,染铅组大鼠各周体重均较低,逃避潜伏期长,血铅含量升高,Glu和Gln含量低,Glu/GABA比值下降(P<0.05);与染铅组比较M-PAS组逃避潜伏期短,L、M和HPAS组海马Glu和Gln含量升高,H-PAS组海马GABA含量降低,L-、M-和H-PAS组Glu/GABA比值高(P<0.05)。结论PAS-Na对铅致幼龄大鼠学习记忆能力受损和海马氨基酸类神经递质异常改变具有一定的拮抗作用。
Objective To investigate the effects of sodium salicylate (PAS-Na) on learning and memory impairment and amino acid neurotransmitters in hippocampus of young rats induced by lead. Methods Fifty male and female SD rats were randomly divided into control group, lead group, low, medium and high dose PAS-Na treatment group (L-, M-, H-PAS). The rats in the lead, L-, M- and H-PAS groups were intraperitoneally injected with 15 mg / kg lead acetate and the control group ip with the same volume of normal saline once a day for 2 weeks. Then, 100, 200 and 300 mg / kg of PAS-Na were injected subcutaneously into the back of the L, M- and H-PAS groups, sc each volume of normal saline on the back of the lead-exposed group and the control group once a day for 3 weeks . The learning and memory abilities of rats were measured by Morris water maze, the content of blood lead was determined by graphite furnace atomic absorption spectrometry and the levels of glutamate, glutamine and glutamine in the hippocampus were determined by HPLC. Acid (GABA) content. Results Compared with the control group, the rats in the lead-exposed group had lower body weight and longer escape latency, higher blood lead levels, lower Glu and Gln levels, and lower Glu / GABA ratios (P <0.05) Compared with M-PAS group, the escape latency was short, the content of Glu and Gln in hippocampus in L, M and HPAS groups was increased, the content of GABA in hippocampus was decreased in H-PAS group, and Glu / GABA ratio was higher in L-, M- and H-PAS groups (P < 0.05). Conclusion PAS-Na can antagonize the impaired learning and memory ability of rats and the abnormal changes of amino acid neurotransmitter in hippocampus of lead-induced aging rats.