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目的自人脑胶质瘤文库克隆新的表达序列标签。方法以人脑胶质瘤文库为模板，利用生长休止特异基因的５′特异引物与载体特异引物组合进行ＰＣＲ扩增，对目的片段进一步克隆、测序。结果扩增出一个３２８ｂｐ的片段，同源性分析发现该片段与鼠ＳＨ２－Ｂ有８６％的同源性。结论ＳＨ２－Ｂ是生长激素信号转导途径中酪氨酸激酶ＪＡＫ２的底物，推测得到的基因片段可能介导胶质瘤的发生、发展过程中的某些信号转导途径，需克隆其ｃＤ－ＮＡ序列并研究其基因表达产物的功能方可证实。
AIM: To clone a new expressed sequence tag from a human glioma library. Methods Human brain glioma library was used as a template to amplify the target fragment by PCR using 5 ’specific primers and carrier specific primers. The target fragment was further cloned and sequenced. As a result, a 328 bp fragment was amplified. Homology analysis revealed that the fragment shared 86% homology with mouse SH2-B. Conclusion SH2-B is a substrate of tyrosine kinase JAK2 in growth hormone signal transduction pathway. It is speculated that the gene fragment may mediate the occurrence and progression of glioma. Some signal transduction pathways need to be cloned -NA sequence and study its function of gene expression products before they can be confirmed.