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目的:研究当归芍药散不同提取部位对体外培养的大鼠海马神经干细胞的增殖作用。方法:以无血清传代培养法,从胎鼠中获得神经干细胞;传至3代后用免疫荧光法对神经干细胞进行鉴定;将当归芍药散不同部位的含药培养基加入神经细胞中进行培养,于第10天进行免疫细胞化学法染色,观察神经干细胞增殖标志物Brdu的表达。结果:当归芍药散的醇提部位(浓度为50μg/ml,100μg/ml)、二氯甲烷部位(浓度为100μg/ml)对体外培养的神经干细胞有明显的增殖作用(P<0.05),正丁醇部位和剩余水部位对体外培养的神经干细胞无明显增殖作用。结论:当归芍药散的醇提部位和二氯甲烷部位可显著促进体外培养大鼠海马神经干细胞的增殖。
Objective: To study the proliferation of rat hippocampal neural stem cells cultured in vitro with different extraction sites of Danggui Shaoyao San. Methods: Neural stem cells were obtained from fetal rats by serum-free subculture method. Neural stem cells were identified by immunofluorescence after passage 3 passage. The drug-containing medium from different parts of Danggui Shaoyao Powder was added to nerve cells for culture, Immunocytochemical staining was performed on the 10th day to observe the expression of neural stem cell proliferation marker Brdu. Results: The ethanol extracts of Danggui Shaoyao San (concentration 50μg / ml, 100μg / ml) and dichloromethane (100μg / ml) had significant proliferative effects on neural stem cells cultured in vitro (P <0.05) Butanol and remaining water had no significant proliferative effects on neural stem cells cultured in vitro. Conclusion: Danggui Shaoyao San alcohol extraction site and methylene chloride can significantly promote the proliferation of rat hippocampal neural stem cells cultured in vitro.