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目的:测定和比较两个产地不同生长时间灯心草皮部和髓部中去氢厄弗酚(DHE)的含量。方法:采用高效液相色谱(HPLC),Agilent Eclipse Plus-C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-水(57∶43),流速1.0 mL·min-1,检测波长270 nm,柱温30℃。结果:灯心草髓部中DHE的含量均明显高于同期皮部;两产地灯心草髓部中去氢厄弗酚的含量,在生长后期差异增大;两产地各时期灯心草皮部中去氢厄弗酚的含量较低,但在灯心草全草中,皮部所含DHE量与髓部相当。结论:H省和J省灯心草中DHE含量差异较大。
OBJECTIVE: To determine and compare the content of dehydroarbenfat (DHE) in the rind and pith of the rutaceae at two different growing stages. Methods: The mobile phase was methanol-water (57:43), the flow rate was 1.0 mL · min-1 using high performance liquid chromatography (HPLC) with Agilent Eclipse Plus-C18 column (4.6 mm × 250 mm, nm, column temperature 30 ℃. Results: The contents of DHE in the stem of Radix Hedysarum were significantly higher than those of the same period. The content of dehydroaverformin in Radix Hedysarum from two producing areas increased significantly in the later period of growth. The content of phenol is low, but in the whole plant of rushes, the amount of DHE contained in the dermis is similar to that in the pulp. Conclusion: The content of DHE in rhamnaceae of H province and J province is quite different.