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以拟南芥ZIF1基因序列为基础,利用生物信息学软件及网站,找出毛果杨中ZIF1基因同源序列,对其进行生物信息学分析,并与其他物种中ZIF1同源基因序列进行多重比对与进化分析,使用实时荧光定量PCR技术验证毛果杨中ZIF1同源基因在锌胁迫条件下,根和叶中该基因的表达情况。结果预测出4个符合ZIF1基因基本结构特征,且具有与该基因相同保守功能结构域的毛果杨ZIF1同源基因,这4个基因属于稳定蛋白,可能具有信号肽,明显疏水区和典型跨膜区,基因定位在质膜上,实时荧光定量PCR结果表明,锌过量存在时,这4个基因表达上调,且根中最为显著。以上分析为进一步研究林木中ZIF1同源基因奠定了理论和实践基础。
Based on the Arabidopsis ZIF1 gene sequence, bioinformatics software and Web site were used to identify the homologous sequences of ZIF1 gene in Populus trichocarpa, followed by bioinformatics analysis and multiple ZIF1 homologous gene sequences in other species Real-time quantitative PCR was used to verify the expression of ZIF1 homologue in Populus trichocarpa under zinc stress. Results Four Zoysia jackii ZIF1 homologues, which conformed to the basic structural characteristics of ZIF1 gene and had the same conserved functional domain, were predicted. These four genes belong to stable protein and may have signal peptide, obvious hydrophobic region and typical cross The membrane and gene were located on the plasma membrane. The results of real-time fluorescence quantitative PCR showed that the expression of these four genes was up-regulated in the presence of zinc excess and the most significant in the roots. The above analysis laid the theoretical and practical basis for further study of ZIF1 homologous genes in trees.