[Objective] To establish a method for determining the contents of hyperoside in ethanolic extract of Abelmoschus manihot (L.) Medic, and study its growth inhibitory effect on Hela cells. [Method] Using 80% ethanol as solvent for extracting total flavonoids, the contents of hyperoside in different parts of Abelmoschus manihot (L.) Medic were determined by HPLC. The HPLC conditions were: chromatographic column of Yilite C18 column (4.6 mm ×250 mm, 5 μm), mobile phase of methanol and 0.05% phosphoric acid solution (V/V, 60∶40), flow rate of 1.0 ml/min, column temperature of 25 ℃, detection wavelength of 360 nm and sampling volume of 10 μl. Growth inhibitory effect of ethanolic extract on Hela cells was assessed by MTT assay. [Results] The hyperoside presented a good linear relationship with peak area in the range of 0.04 to 0.4 mg/ml with correlation coefficient at 0.999 8. The content of hyperoside decreased in the following order, flowers, leaves, stems, roots and seeds. The average content in flowers was the highest that up to 4.002%. The average recovery and RSD value were 99.23% and 1.08% (n=6), respectively. Flavonoids extract of Abelmoschus manihot (L.) Medic could effectively suppress the growth of Hela cells, IC50 of which was 228 μg/ml. [Conclusion] The method was accurate, simple and reproducible that could be used for determining the hyperoside in Abelmoschus manihot (L.) Medic. The flavonoids extract was provided with strong growth inhibitory effect on Hela cells. [Objective] To establish a method for determining the content of hyperoside in ethanolic extract of Abelmoschus manihot (L.) Medic, and study its growth inhibitory effect on Hela cells. [Method] Using 80% ethanol as solvent for extracting total flavonoids, the contents of hyperoside in different parts of Abelmoschus manihot (L.) Medic were determined by HPLC. The HPLC conditions were: chromatographic column of Yilite C18 column (4.6 mm × 250 mm, 5 μm), mobile phase of methanol and 0.05% phosphoric acid Growth inhibitory effect of ethanolic extract on Hela cells was assessed by MTT (V / V, 60:40), flow rate of 1.0 ml / min, column temperature of 25 ° C, detection wavelength of 360 nm and sampling volume of 10 μl. assay. [Results] The hyperoside presented a good linear relationship with peak area in the range of 0.04 to 0.4 mg / ml with correlation coefficient at 0.999 8. The content of hyperoside decreased in the following order, flowers, leaves, stems, roots and seeds. The aver age content in flowers was the highest that up to 4.002%. The average recovery and RSD value were 99.23% and 1.08% (n = 6), respectively. Flavonoids extract of Abelmoschus manihot (L.) Medicient could suppress the growth of Hela The IC50 of which was 228 μg / ml. [Conclusion] The method was accurate, simple and reproducible that could be used for determining the hyperoside in Abelmoschus manihot (L.) Medic. The flavonoids extract was provided with strong growth inhibitory effect on Hela cells.