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目的探讨酶标法检测抗菌素残留量的数据处理方法。方法采用卡那霉素和庆大霉素快速检测试剂盒(间接竞争ELISA法)检测乙型脑炎减毒活疫苗2种抗菌素的残留量;采用半对数直线、半对数二次曲线和对数直线3种方法,以最小二乘法对实验数据进行拟合处理,分析、比较实验点残差分布、残差平方和、相关系数和实验误差等指标。结果半对数直线拟合较差,半对数二次曲线和对数直线方程拟合较好;半对数二次曲线法中实验点在标准曲线两侧的分布较均匀,各点的残差平方和远小于半对数直线法;半对数二次曲线法和对数直线法所得标准曲线的相关系数较高,检测庆大霉素标准品的实验误差均小于半对数直线法。结论半对数二次曲线法和对数直线法均优于试剂盒推荐的半对数直线法,而在极限值处理、计算简便等方面对数直线法更优。
Objective To investigate the data processing method of enzyme-labeled method for the determination of antibiotic residues. Methods The kanamycin and gentamicin rapid detection kit (indirect competitive ELISA) was used to detect the residues of two antibiotics in live attenuated JE vaccine. The semi-logarithmic and semi-logarithmic quadratic curves Logarithmic straight line three methods, the least square method of fitting experimental data, analysis, comparison experimental point residual distribution, residual sum of squares, correlation coefficient and experimental error and other indicators. Results The semi-logarithmic straight line fitted poorly, and the semi-logarithmic quadratic curve and logarithmic straight line fit well. The experimental points in semi-logarithmic conic curve were more evenly distributed on both sides of the standard curve, The difference square sum is much less than the semi-logarithmic straight line method; the correlation coefficient between the semi-logarithmic quadratic curve method and the logarithmic straight line method is higher, and the experimental error of detecting gentamycin standard samples is less than the semi-logarithmic straight line method. Conclusions Semi-logarithmic quadratic curve method and logarithmic normal method are better than the semi-logarithmic straight line method recommended by the kit, but logarithmic linear method is superior in terms of limit value calculation and simple calculation.