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为了了解生精上皮内物质的代谢过程,4种针对大鼠溶酶体膜蛋白的单克隆抗体(Barrio et al,1986a,b)通过免疫酶标(ABC)法被用来标记了生精上皮内的4种溶酶体膜蛋白,其中初级精母细胞的溶酶体膜蛋白最丰富且围绕着核的四周;随着精细胞的成熟与变态,其溶酶体膜蛋白显示溶酶体逐渐偏居胞核一侧并移向精子的尾部。此结果提示:(1)在生精上皮中溶酶体膜蛋白由多种蛋白所构成,其中各种蛋白的含量是不同的,(2)在生精上皮内精母细胞的合成与分解代谢最旺盛,其细胞的自溶作用也最强;(3)随着精细胞的成熟,细胞内的合成与分解代谢逐渐降低,其溶酶体的量逐渐减少。
To understand the metabolic processes of spermatogenic epithelial substances, four monoclonal antibodies to rat lysosomal membrane proteins (Barrio et al., 1986a, b) were used to label the seminiferous epithelium by immunoblot (ABC) Within the four lysosomal membrane proteins, of which the primary spermatocyte lysosomal membrane protein is most abundant and around the nucleus; with the sperm maturation and metamorphosis, the lysosomal membrane proteins showed that lysosomes gradually Deviate from the nucleus side and move to the sperm tail. The results suggest that: (1) in the seminiferous epithelium, lysosomal membrane proteins consist of a variety of proteins, the contents of which are different, (2) the synthesis and catabolism of spermatocytes in the seminiferous epithelium The strongest, the autolysis of its cells is also strongest; (3) With the sperm maturation, the synthesis and catabolism of cells gradually decreased, the amount of lysosomes gradually reduced.