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运用染色体原位显带方法,观察和分析26例食管癌患者和45例正常人淋巴细胞在常规培养条件下,自发和诱发(互隔交链孢霉提取物C12b3-2、MNNG)的染色体断裂热点(即频发断裂点)与脆性部位、肿瘤相关断裂点和癌基因位点的相关性。结果表明食管癌患者自发断裂点与脆性部位和肿瘤相关断裂点明显相关,提示自发断裂热点可能与该患者对肿瘤的易感性高有关;同时正常人的自发热点与脆性部位和肿瘤相关断裂点均不相关,可能与正常人对肿瘤易感性低有关。实验结果表明C12b3-2和MNNG诱发的新断点与肿瘤相关断点表现出明显相关,提示这两种致癌物的致癌作用可能与其对肿瘤相关断裂点的特异性有关。但无论是自发还是诱发断裂热点均不与癌基因位点相关。
Chromosome rupture was observed and analyzed in 26 cases of esophageal cancer patients and 45 cases of normal human lymphocytes under routine culture conditions using chromosomal in situ banding method Hot spots (ie, frequent break points) are associated with sites of fragility, tumor-associated breakpoints, and oncogenes. The results showed that spontaneous rupture of esophageal cancer patients with fragile sites and tumor-related fracture point was significantly correlated, suggesting that spontaneous rupture of hot spots may be related to the high susceptibility of the tumor; the same time, normal spontaneous hot spots and brittle parts and tumor-related breakpoints Irrelevant, may be related to normal and low tumor susceptibility. The experimental results show that the new breakpoints induced by C12b3-2 and MNNG are significantly correlated with tumor-related breakpoints, suggesting that the carcinogenicity of these two carcinogens may be related to their specificity for tumor-associated breakpoints. However, neither spontaneous nor induced hot spots were associated with oncogene loci.