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以苦丁茶树腋芽为外植体进行培养，诱导腋芽萌发，产生丛芽，同时，试管内外结合法诱导生根，并大田移栽．结果表明：ａ．初代培养以Ｂ５＋ＢＡ２ｍｇ／Ｌ＋ＩＢＡ０．５ｍｇ／Ｌ＋ＧＡ３０．５ｍｇ／Ｌ固体培养基诱导腋芽萌发较好；ｂ．Ｂ５＋ＢＡ０．５ｍｇ／Ｌ＋ＺＴ０．２ｍｇ／Ｌ＋ＧＡ３０．２ｍｇ／Ｌ固体培养基能诱导大量丛芽产生；ｃ．适当剪除叶片并去掉顶芽能促进腋芽萌发；ｄ．小苗在附加ＮＡＡ０．２ｍｇ／Ｌ，ＩＢＡ０．２ｍｇ／Ｌ，或ＮＡＡ０．１ｍｇ／Ｌ，ＩＢＡ０．２ｍｇ／Ｌ的１／２ＭＴ，１／２Ｂ５培养基上培养１０～１５ｄ，插入蛭石上发根，成苗快；ｅ．土壤保湿、避免强光照，是小苗成活的关键．
The axillary buds of Ilex kudingcha as explants were cultured to induce axillary buds to germinate and produce cluster buds. At the same time, rooting was induced in vitro and in vitro by test tube and transplanted in field. The results show that: a. Primary culture with B5 + BA2mg / L + IBA0.5mg / L + GA30.5mg / L solid medium induced axillary bud germination is better; b. B5 + BA0.5mg / L + ZT0.2mg / L + GA30.2mg / L solid medium can induce a large number of buds; c. Appropriate pruning leaves and remove the top buds can promote axillary buds germination; d. The seedlings were cultured on medium supplemented with NAA0.2mg / L, IBA0.2mg / L, NAA0.1mg / L, IBA0.2mg / L in1 / 2MT and1 / 2B5 medium for10-15d, Miao fast; e. Soil moisture, avoid strong light, is the key to survival of seedlings.