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为了探讨鼠脑缺血后脑内组织型纤溶酶原激活物 ( t PA)及其主要抑制物 - 1型 ( PAI- 1)蛋白的表达。制备了大鼠全脑和局灶性脑缺血模型 ,用免疫组化法检测 t PA和 PAI- 1蛋白的表达。结果发现 ,在全脑、局灶性脑缺血不同时间和正常鼠脑膜、脉络膜、室管膜、血管内皮等部位均有 t PA、PAI- 1免疫阳性信号 ,但小脑、海马区未见该信号。局灶性脑缺血 6 h,缺血侧中心部位的胶质细胞和梗死灶周边的缺血半影区的神经元也显示 t PA强阳性信号 ,但该细胞不表达 PA I- 1蛋白 ;缺血 18h鼠缺血侧未见t PA、PAI- 1阳性信号。本研究提示 ,脑缺血后鼠脑组织和正常脑组织均有 t PA、PA I- 1蛋白表达 ,但全脑缺血后和正常对照鼠t PA蛋白表达无明显变化 ,而局灶性脑缺血 6 h鼠缺血侧比正常侧 t PA蛋白表达增高 ,脑缺血后鼠脑组织和正常鼠 PA I- 1蛋白表达无显著变化
In order to investigate the expression of tissue-type plasminogen activator (t PA) and its major inhibitor of type 1 (PAI-1) in rat brain after cerebral ischemia. Rat models of whole brain and focal cerebral ischemia were prepared, and the expressions of t PA and PAI-1 protein were detected by immunohistochemistry. The results showed that tPA and PAI-1 immunoreactive signals were detected in the whole brain, focal cerebral ischemia and normal rat brain, choroid, ependymal membrane and vascular endothelium. However, no signal was observed in cerebellum and hippocampus signal. After 6 h of focal cerebral ischemia, the neurons in glial cells in ischemic center and ischemic penumbra surrounding infarct also showed strong positive signal of t PA, but the cells did not express PA I-1 protein. Ischemia 18h ischemia rat t PA, PAI-1 positive signal. This study suggests that t PA, PA I-1 protein expression in brain tissue and normal brain tissue after cerebral ischemia, but there was no significant change in t PA protein expression after global cerebral ischemia and normal control rats, and focal brain The expression of t PA protein increased at 6 h after ischemia in the ischemic and the PA I-1 protein expression in brain tissue and normal rats after cerebral ischemia