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采用高效液相色谱-蒸发光散射检测法测定木薯中果糖、葡萄糖、蔗糖和海藻糖的含量。样品用水经超声波提取,用Xbridge-NH_2色谱柱分离,流动相为乙腈∶水(水中加入0.2%的三乙胺)的体积比为70:30,流速为1.0m L/min,Elsd漂移管温度为85℃,氮气流速2 L/min。在该条件下果糖、葡萄糖、蔗糖和海藻糖得到很好的分离,分别在1.5~25,1.5~25,2~25和1.5~25μg的范围内有良好的线性范围,相关系数R~2分别为0.999 1,0.999 1,0.999 0和0.999 3,最低检出限分别为6.50,5.50,4.00和7.00 mg/kg,加标回收率分别为97.6%,98.6%,96.2%和95.4%。验证试验结果表明,该方法快速、准确,能用来测定木薯中单糖的含量。
The content of fructose, glucose, sucrose and trehalose in cassava was determined by high performance liquid chromatography with evaporative light scattering detection. The sample was extracted with water by ultrasonic wave and separated by Xbridge-NH 2 column. The mobile phase consisted of 70:30 volume ratio of acetonitrile: water (0.2% triethylamine in water), flow rate of 1.0 m L / min, Elsd drift tube temperature 85 ° C, nitrogen flow 2 L / min. Under these conditions, fructose, glucose, sucrose and trehalose were well separated and had good linear range in the range of 1.5-25, 1.5-25, 2-25 and 1.5-25 μg, respectively, and the correlation coefficients R 2 With the lowest detection limits of 6.50, 5.50, 4.00 and 7.00 mg / kg, respectively. The recoveries were 97.6%, 98.6%, 96.2% and 95.4%, respectively. Validation test results show that the method is rapid and accurate and can be used to determine the content of monosaccharides in cassava.