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目的:采用HPLC法测定温阳活血颗粒中芍药苷、人参皂苷Rg_1、Rb_1、Re的量。方法:色谱柱Agilent TC-C18(250 mm×4.6 mm,5μm),芍药苷流动相为乙腈-0.1%磷酸水溶液(14∶86),检测波长为230 nm,流速1.0 m L/min;人参皂苷Rg_1、Rb_1、Re流动相为流动相A(乙腈)-B(水)梯度洗脱(0 min 19%A,35 min 19%A,55 min 29%A,70 min 29%A,100 min 40%A),漂移管温度为60℃,雾化气体为高纯氮气,Gain 6,氮气压力3.0MPa,流速1.0 m L/min。结果:芍药苷在0.402~4.02μg线性关系良好,r=0.999 7,平均加样回收率为98.15%,RSD为2.07%。人参皂苷Rg_1、Re、Rb_1分别在0.445~4.45μg(r=0.999 7)、0.464~4.64μg(r=0.999 6)、0.647~6.47μg(r=0.999 8)线性关系良好,平均加样回收率分别为98.23%、97.36%、97.58%,RSD分别为2.21%,2.17%,2.09%。结论:该方法测定结果准确可靠、灵敏度高、重复性好,可用于本制剂的质量控制。
Objective: To determine the contents of paeoniflorin, ginsenoside Rg_1, Rb_1 and Re in Wenyang Huoxue Granules by HPLC. METHODS: The mobile phase of paeoniflorin was acetonitrile-0.1% phosphoric acid solution (14:86) with a detection wavelength of 230 nm and a flow rate of 1.0 m L / min on a column Agilent TC-C18 (250 mm × 4.6 mm, (0 min 19% A, 35 min 19% A, 55 min 29% A, 70 min 29% A, 100 min 40) with mobile phase Rg_1, Rb_1, % A). The temperature of the drift tube was 60 ℃. The atomization gas was high purity nitrogen, Gain 6, nitrogen pressure 3.0 MPa, flow rate 1.0 m L / min. Results: Paeoniflorin had a good linearity between 0.402 and 4.02μg, r = 0.999 7, the average recovery was 98.15% and the RSD was 2.07%. Ginsenosides Rg_1, Re and Rb_1 showed good linearity in the range of 0.445-4.45μg (r = 0.999 7), 0.464-4.46μg (r = 0.999 6) and 0.647-6.47μg (r = 0.999 8) Respectively, 98.23%, 97.36%, 97.58%, RSD respectively 2.21%, 2.17%, 2.09%. Conclusion: The method is accurate, reliable, sensitive and reproducible. It can be used for the quality control of this preparation.