一、疫苗失效。一是过了有效期;二是保存温度过高,疫苗自制造之日算起,在5—8℃冰箱内保存,有效期为一年,在15—25℃的室温下三个月就失效;三是用热开水或温水稀释疫苗;四是因当天未用完,放到隔天再用;五是把稀释后的疫苗暴露在阳光下然后再接种;六是疫苗使用前,胶塞破碎或玻璃破裂、色泽不正常等情况失效而致。二、接种疫苗时,违反操作规程。如注 First, the vaccine fails. One is over a valid period; the second is to save the temperature is too high, the vaccine since the date of manufacture, stored in 5-8 ℃ refrigerator, valid for one year, three months at 15-25 ℃ room temperature failure; three Is to use hot boiled water or warm water to dilute the vaccine; Fourth, because the day did not run out, put into use the next day; Fifth, the diluted vaccine is exposed to the sun and then vaccinated; Six is ​​the vaccine before use, Rupture, color is not normal and so on failure. Second, the vaccination, in violation of rules. If note