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用杨树杂种,日本山杨×大齿杨(Populus Sieboldii X P.grandidentata)3株,马氏杨(P.Maximowiczii)和美州黑杨(P.deltoides)雄株各一株,研究了花药诱导愈伤组织和生长调节物质对器官分化的效果。基本培养基采用了WOLTER和SKOOG培养基(WS——培养基)和MILLER培养基(M——培养基),生长素采用了NAA(萘乙酸),IBA(吲哚丁基酸)、2.4——D(二氯苯基乙酸),细胞分裂素(cytokinin)采用了BAP(N～6——benzylaminopurine,苄胺基嘌哈),组合成各种浓度加进去了。得到的结果如下: 1)植物生长素类和BAP混合使用对愈伤组织诱导的效果-对杨树杂种只是在低浓度
Populus Sieboldii × P.grandidentata, Populus Sieboldii × P.grandidentata, P. maimimowiczii and P.deltoides were used to study the anther induction Effects of callus and growth regulators on organ differentiation. Essential medium used WOLTER and SKOOG medium (WS - medium) and MILLER medium (M - medium), auxin used NAA (naphthalene acetic acid), IBA (indole butyl acid), 2.4- -D (dichlorophenylacetic acid), cytokinin using BAP (N ~ 6 - benzylaminopurine, benzylamine purine), combined into various concentrations added. The results obtained are as follows: 1) The effect of the combination of auxin and BAP on callus induction - for poplar hybrids only at low concentrations