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基于保泰松对联吡啶钌的电致化学发光信号有较强的增敏作用,使用多壁碳纳米管修饰玻碳电极作为工作电极,建立了一种保泰松的电致化学发光检测方法.对测定条件进行了一系列优化,结果表明,当发光试剂联吡啶钌的浓度为1.0×10-5mol/L时,在0.05mol/LpH10.20的Na2HPO4-NaOH介质中保泰松对联吡啶钌电化学发光信号的增敏效果最强.在此条件下,保泰松的线性范围为0.5～200μmol/L,检测限(S/N=3)为0.2μmol/L,与使用未经修饰的裸玻碳电极相比,检测限下降了约一个数量级.该检测方法还被用于加标人血清样品中保泰松的测定,回收率在85.3%～95.0%之间,结果令人满意,可望用于保泰松的药物临床分析与质量监控.
Based on the strong sensitizing effect of phenylbutazone to the electrochemiluminescent signal of bipyridyl ruthenium, a multi-walled carbon nanotube modified glassy carbon electrode was used as the working electrode to establish a method for the determination of phenylbutazone by electrochemiluminescence. The results showed that when the concentration of luminescent reagent bipyridyl ruthenium was 1.0 × 10-5mol / L, the effect of Phenylbutazone on ruthenium bipyridine bipyridine in Na2HPO4-NaOH medium of 0.05mol / LpH10.20 Under these conditions, the linear range of phenylbutazone was 0.5 ~ 200μmol / L, the detection limit (S / N = 3) was 0.2μmol / L, which was the same as that of the unmodified Compared with glassy carbon electrode, the detection limit decreased by about one order of magnitude.The detection method was also applied to the determination of phenylbutazone in spiked human serum samples with the recoveries between 85.3% and 95.0% with satisfactory results. Prospect for the drug Phenylbutazone clinical analysis and quality control.