论文部分内容阅读
目的:研究中药柴芍承气汤对大鼠肠缺血再灌注(I/R)继发肝损伤的保护作用及其机制。方法:30只大鼠分为3组,对照组、模型组和中药组各10只。通过免疫组织化学染色方法、Western blot和RT-PCR法检测大鼠肝组织半胱胺酰白三烯受体1(cysteinyl leukotriene receptor 1,Cys LTR1)蛋白和mRNA表达水平,并用光学显微镜观察肝脏病理改变,TUNEL方法检测肝细胞凋亡。结果:(1)与对照组比较,模型组有大鼠肝脏病理损伤,中药组大鼠组肝脏组织的病理改变明显减轻(均P<0.05);(2)肠I/R时肝组织Cys LTR1蛋白和mRNA表达水平升高;应用中药后,肝组织Cys LTR1蛋白和mRNA表达水平均减低(均P<0.05);(3)与对照组比较,模型组肝细胞凋亡指数升高;与模型组比较,中药组肝细胞凋亡显著减少(P<0.01);(4)肝组织中Cys LTR1蛋白表达分别与细胞凋亡、肝脏组织病理损伤正相关(r=0.8463、0.8783、0.7708,均P<0.05)。结论:柴芍承气汤能减轻肠I/R继发肝脏损伤的病理损害和降低肝细胞凋亡,并能抑制肝脏组织中Cys LTR1的表达,柴芍承气汤可能通过抑制肝脏组织中Cys LTR1的表达和抑制肝细胞凋亡减轻肠I/R继发肝损伤。
Objective: To study the protective effect of Chai Ju Cheng Cheng Qi Decoction on hepatic injury induced by intestinal ischemia-reperfusion (I / R) in rats and its mechanism. Methods: Thirty rats were divided into three groups: control group, model group and TCM group. The protein and mRNA expressions of cysteinyl leukotriene receptor 1 (Cys LTR1) were detected by immunohistochemical staining, Western blot and RT-PCR. The pathological changes of liver were observed by light microscopy Change, TUNEL method to detect hepatocyte apoptosis. Results: (1) Compared with the control group, the model group had pathological changes of liver in rats, and the pathological changes in the liver tissue of the TCM group were relieved (all P <0.05). (2) The Cys LTR1 (P <0.05). (3) Compared with the control group, the apoptotic index of hepatocyte in the model group increased, and the expression of Cys LTR1 protein and mRNA in the liver tissue of the model group decreased significantly (P <0.01). (4) The expression of Cys LTR1 protein in liver tissue was positively correlated with apoptosis and liver histopathological damage (r = 0.8463,0.8783,0.7708, P <0.05). Conclusion: Chai Sha Cheng Cheng Qi Decoction can reduce pathological damage secondary to intestinal I / R injury and reduce hepatocyte apoptosis, and can inhibit the expression of Cys LTR1 in liver tissue, Chai Chai Cheng Qi Decoction may inhibit liver Cys LTR1 expression and inhibition of hepatocyte apoptosis reduce intestinal I / R secondary liver injury.