论文部分内容阅读
目的：探讨细胞增殖与程序性细胞死亡在颞下颌关节发育中的作用。方法：利用免疫组织化学技术及原位末端标记法，对胚胎及生后一周ＳＤ大鼠颞下颌关节不同发育时期髁状突软骨中增殖细胞核抗原（ＰＣＮＡ）的表达及程序性细胞死亡（ＰＣＤ）进行了观察。结果：胎鼠颞下颌关节髁状突软骨增殖层ＰＣＮＡ阳性细胞率最高，进入浅层肥厚层ＰＣＮＡ阳性细胞数减少，深层肥厚层中则未见ＰＣＮＡ阳性细胞。出生后髁状突软骨ＰＣＮＡ阳性细胞率均较出生前低。ＰＣＤ阳性细胞主要分布于软骨增殖层及邻近深层肥厚层的浅层肥厚层中，在关节腔开始形成时髁状突软骨表面ＰＣＤ明显。结论：细胞增殖与程序性细胞死亡密切相关，协同参与调控颞下颌关节的生长发育与塑形。
Objective: To investigate the role of cell proliferation and programmed cell death in the development of temporomandibular joint. Methods: The expression of proliferating cell nuclear antigen (PCNA) and programmed cell death (PCD) in condylar cartilage during different developmental stages of the temporomandibular joint of SD rats were detected by immunohistochemistry and in situ end labeling. Observed. RESULTS: The PCNA positive cells of condylar cartilage proliferating layer in fetal temporomandibular joint were the highest, while the number of PCNA positive cells in the superficial layer of hypertrophic layer decreased and no PCNA positive cells were found in the deep layer of hypertrophic layer. Postnatal condylar cartilage PCNA positive cells were lower than before birth. PCD positive cells were mainly distributed in the superficial hypertrophic layer in the cartilage proliferation layer and the adjacent deep hypertrophic layer, and the PCD of the condylar cartilage surface was obvious when the joint cavity began to form. Conclusion: Cell proliferation is closely related to programmed cell death and synergistically participates in the regulation of temporomandibular joint growth and development.