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以往用于研究角蛋白的免疫电镜技术一直是包埋前染色法,或用低温包埋剂包埋后染色法。前者仅允许有限的抗体渗透入组织;后者则不仅需要特殊的设备,而且对细胞结构有破坏,并需用新鲜组织。本文用常规固定和包埋材料,用蛋白 A-胶体金包埋后染色法标记角蛋白,结果表明这种免疫电镜技术是一种非常有用的方法,组织结构形态保存良好,而且包埋好的组织块可以用于回顾性研究。作者用两块常规电镜诊断标本,经常规固定、乙醇梯度脱水、环氧树脂包埋,将表皮超薄切片置于200目镍网,过碘酸钠饱和溶液浸蚀1小时,双蒸水冲洗,1%牛血清白蛋白(BSA)中室温10～15分钟,在抗人角蛋白抗体中(1∶1000稀释于
In the past for the study of keratin immunoelectron microscopy has been embedded before staining, or buried with low temperature embedding staining. The former allows only a limited amount of antibody to penetrate the tissue; the latter requires not only special equipment but also disruption of the cell structure and the need for fresh tissue. In this paper, conventional immobilization and embedding materials, protein A-colloidal gold staining after labeling keratin, the results show that this kind of immunoelectron microscopy is a very useful method, well-preserved organizational structure, and well-embedded Tissue blocks can be used for retrospective studies. The authors used two routine electron microscopic diagnosis of specimens, routine fixed ethanol dehydration, epoxy resin embedded in ultra-thin epidermis placed 200 mesh nickel net, saturated sodium periodate solution for 1 hour, double distilled water rinse , 1% bovine serum albumin (BSA) for 10-15 minutes at room temperature, in anti-human keratin antibodies (1: 1000 diluted in