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肾小管损伤病人尿中低分子量(LMW)蛋白,如MW 11,800的β_2-微球蛋白和MW21,400的维生素A结合蛋白明显增多,镉中毒病人亦见有LMW蛋白尿,故将尿中LMW蛋白测定列为镉性肾小管损伤的筛选指标。为了弄清尿中LMW增加是否为肾小管损伤所致,并鉴别不同的肾毒性化合物引起的蛋白尿,进行了下述实验。家兔分别皮下注射氯化镉、醋酸双氧铀、重铬酸钾、氯化汞、顺丁烯二酸和卡那霉素。镉(0.5 mg/kg)每周注射6次,共32周;卡那霉素隔日注射一次,其余各组仅注射一次。注后不同的间隔期测定受检动物尿蛋白,用SDS-PAGE法分析其分子量分布,同时测定体重、尿氨基酸、尿糖、肌
Urine low molecular weight (LMW) protein in patients with renal tubular trauma, such as β 2-microglobulin in MW 11,800 and Vitamin A-binding protein in MW 21,400 increased significantly, and patients with cadmium intoxication also had LMW proteinuria. Therefore, urinary LMW protein Determination as a marker of cadmium tubule injury screening. In order to understand whether the increase in urinary LMW is caused by renal tubular damage and to identify proteinuria caused by different nephrotoxic compounds, the following experiment was performed. Rabbits were subcutaneously injected with cadmium chloride, uranyl acetate, potassium dichromate, mercuric chloride, maleic acid and kanamycin. Cadmium (0.5 mg / kg) was injected six times per week for 32 weeks; kanamycin was injected every other day, and the remaining groups were injected only once. At different intervals, the urinary protein of test animals was measured, and its molecular weight distribution was analyzed by SDS-PAGE. Body weight, urine amino acids, urine glucose, muscle