美国的Simons等[1]、Leary等[2]和Linnen等[3]分别从肝炎病人的血清中克隆出两株GBV－C病毒和一株庚型肝炎病毒（HGV），并确定立者为同一病毒的不同亚型病毒，这一新型肝炎病毒的确定为非甲-戊型肝炎的病原学诊断提示了新思路。我们采用逆转录一套式一聚合酶链反应（RT－neste Simons et al [1], Leary et al [2] and Linnen et al [3] separately cloned two strains of GBV-C and one strain of hepatitis G virus (HGV) from the sera of hepatitis patients and determined that Identification of different subtypes of the same virus, the identification of this new type of hepatitis virus, suggests a new idea for the etiological diagnosis of non-E-hepatitis. We used RT-nested polymerase chain reaction (RT-neste