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目的探讨最敏感的ＨＢＶ垂直传播的检测指标。方法本文应用ＥＬＩＳＡ法和ＰＣＲ技术检测了５８位妊娠孕妇及其新生儿血清ＨＢＶ标志物（ＨＢＶｍ）与ＨＢＶＤＮＡ。结果ＨＢｓＡｇ阳性与阴性孕妇的新生儿ＨＢＶＤＮＡ检出率分别为５５．８％和５．０％。两者间差异有显著性（Ｐ＜０．００５），ＨＢＶＤＮＡ阳性与阴性孕妇的新生儿ＨＢＶＤＮＡ检出率分别为６８．８％和０，说明检测孕妇血ＨＢＶＤＮＡ，可提高新生儿血ＨＢＶＤＮＡ的检出率。所有新生儿ＨＢＶｍ和ＨＢＶＤＮＡ阳性率分别为１２．０７％和３７．９％。结论提示检测新生儿血清ＨＢＶｍ难以排除宫内感染，ＰＣＲ法可作为子宫内感染指标，或者ＨＢＶｍ与ＨＢＶＤＮＡ同时检测以早期诊断新生儿宫内感染以便尽早防治。
Objective To explore the most sensitive detection of HBV vertical transmission. Methods The serum HBV markers (HBVm) and HBVDNA in 58 pregnant women and their newborns were detected by ELISA and PCR. Results The positive rates of HBVDNA in HBsAg positive and negative pregnant women were 55.8% and 5.0% respectively. The difference between the two groups was significant (P <0.005), HBVDNA positive and negative pregnant women HBVDNA positive rate of 68.8% and 0, respectively, indicating that the detection of blood HBV DNA in pregnant women, can improve neonatal blood HBVDNA test Rate out. The positive rates of HBVm and HBVDNA in all newborns were 12.07% and 37.9% respectively. Conclusions The detection of neonatal serum HBVm is difficult to rule out intrauterine infection, PCR method can be used as an indicator of intrauterine infection, or HBVm and HBVDNA simultaneous detection of early diagnosis of neonatal intrauterine infection in order to prevent and cure as soon as possible.