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利用辐射能量为80 MeV/u的12C6+重离子束辐照番茄种子,辐照剂量分别为30,60,90,120和160 Gy研究其对番茄M1代的生物学效应。结果表明,随着辐照剂量的增大,番茄的发芽率和成苗率降低,且成苗率明显低于发芽率,发现辐照损伤主要抑制了根的生长;MDA和脯氨酸含量变化的总体趋势为随着辐照剂量的增大先升后降再升高,说明高剂量C离子辐照对生物膜造成更严重的损伤;APX活性随着辐照剂量的增大呈先升后降再升高的趋势,表明APX在清除活性氧中起主要作用;POD和SOD活性的总体趋势是随着辐照剂量的增大而降低,且明显低于对照组。综合分析表明,12C6+重离子束辐照番茄种子,对M1代具有明显的损伤效应,高剂量辐照对番茄种子造成的损伤更大,使酶的活性降低,抑制植株生长。
The tomato seeds were irradiated with 12C6 + heavy ion beam with a radiation energy of 80 MeV / u, and the biological effects on the M1 generation of tomato were studied at irradiation doses of 30, 60, 90, 120 and 160 Gy respectively. The results showed that with the increase of irradiation dose, the germination rate and seedling formation rate of tomato decreased, and the seedling emergence rate was significantly lower than that of germination rate. The results showed that the radiation damage mainly inhibited the root growth; the content of MDA and proline The general trend was that the radiation dose increased first and then decreased then increased, indicating that high doses of C irradiation caused more serious damage to the biofilm; APX activity increased with the radiation dose increased first And then increased, indicating that APX played a major role in scavenging reactive oxygen species (ROS). The overall trend of POD and SOD activity decreased with the increase of irradiation dose, which was significantly lower than that of control group. Comprehensive analysis showed that 12C6 + heavy ion beam irradiation of tomato seeds had obvious damaging effects on M1 generation. High dose irradiation caused more damage to tomato seeds, reduced enzyme activity and inhibited plant growth.