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人宫颈癌与人乳头瘤病毒(HPV)的关系是当前国际重视的研究课题之一。在我国有关研究鲜有报道。本文在国内五个地区(新疆、北京、湖北、黑龙江、贵州)共获得病理学确诊的人宫颈鳞状上皮癌318例,宫颈上皮内新生物(CIN)14例,宫颈及外阴湿疣48例,慢性宫颈炎34例及正常宫颈上皮组织24例。将上述组织提取DNA后,以~32P标记的HPV—11,16,18三型病毒DNA为探针与上述组织进行Dot Blot及Southern Blot核酸杂交。其中32例宫颈癌10例正常宫颈及27例宫颈及外阴湿疣还进行平行的电子显微镜对比研究。对于湿疣病例还进行了以酶标法代替H标记的原位核酸杂交的观察研究,结果表明:
The relationship between human cervical cancer and human papillomavirus (HPV) is currently one of the most important research topics in the world. Little research has been reported in our country. In this paper, 318 pathologically confirmed squamous cell carcinomas of the cervix, 14 cervical intraepithelial neoplasms (CINs), 48 cervical and genital warts (48 cases) were screened in five regions of China (Xinjiang, Beijing, Hubei, Heilongjiang and Guizhou) Chronic cervicitis in 34 cases and normal cervical epithelium in 24 cases. After the DNA was extracted from the above tissues, Dot Blot and Southern Blot nucleic acid hybridizations were performed with the HPV-11, HPV 16, and HPV 16 DNA probes as probes. 32 cases of cervical cancer in 10 cases of normal cervix and 27 cases of cervical and genital warts also conducted parallel electron microscopy comparative study. For cases of genital warts also carried out by enzyme labeling method instead of H-labeled in situ hybridization of the observed results showed that: