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目的用致癌物四氧二苯二氧杂环已二烯(二,TCDD)和苯并(a)芘(B(a)P)联合诱导构建大鼠肺癌的模型,探讨芳香烃受体(AhR)介导TCDD致肺癌的发生机制。方法选Wistar大鼠80只,随机分为ABCD 4组分别代表施以TCDD、TCDD+B(a)P、B(a)P染毒的处理组和未施任何染毒处理的对照组,各组在第1.5、3、4.5和6个月,分批系列宰杀,观察肺部癌变情况。结果结果表明,TCDD染毒组在101 d时出现首例肺癌,累计TCDD用量865.94 ng,致癌率为15%,TCDD+B(a)P联合染毒组在81 d时出现首例肺癌,TCDD累计用量622.34 ng,B(a)P累计用量为26.83 mg;致癌率30%,B(a)P染毒C组在161 d时,出现首例肺癌,B(a)P累计用量为87.58 mg,致癌率为5%;对照组未出现肺癌。4组之间比较,差异有统计学意义(P<0.05)。结论TCDD和B(a)P成功地诱发了大鼠肺癌,证实了TCDD既是致癌剂又是促癌剂。
OBJECTIVE: To establish a model of lung cancer in rats by combination of carcinogenic tetraphenylnaphthalene dioxane (TCDD) and benzo (a) pyrene (B (a) P) ) Mediates the mechanism of TCDD-induced lung cancer. Methods Eighty Wistar rats were randomly divided into four groups: ABCD, TCDD + B (a) P, B (a) P, and control group without any exposure Groups were slaughtered in batches at days 1, 5, 3, 4.5 and 6 to observe lung canceration. The results showed that the first case of lung cancer was found on the 101th day in TCDD-treated group, with an accumulated TCDD dosage of 865.94 ng and a carcinogenic rate of 15%. TCDD + B (a) The cumulative dosage of B (a) P was 622.34 ng, the cumulative dosage of B (a) P was 26.83 mg; the carcinogenic rate was 30% , Carcinogenic rate was 5%; control group did not appear lung cancer. The differences between the 4 groups were statistically significant (P <0.05). Conclusion TCDD and B (a) P successfully induced lung cancer in rats and confirmed that TCDD is both a carcinogen and a carcinogen.