论文部分内容阅读
【目的】明确茶尺蠖Ectropis obliqua Prout 5龄幼虫脑解剖结构,并分析和构建幼虫脑以及脑内部各神经髓结构的三维结构模型。【方法】采用免疫组织化学方法,利用突触蛋白抗体,染色标记脑内神经突触,定位突触联系密集分布的区域,获得脑内部神经髓的结构。利用激光共聚焦显微镜获取脑扫描图像,然后利用三维图像分析软件AMIRA进行图像分析,构建脑的三维结构模型,并计算脑以及脑内各神经髓结构的体积。【结果】突触蛋白抗体染色显示,茶尺蠖5龄幼虫脑内具有很多神经突触联系密集分布的区域,这些不同区域即为脑的不同神经髓结构。茶尺蠖幼虫脑主要包括前脑、中脑和后脑3个组成部分。其中前脑最大,包括成对的视叶、蕈形体、前脑桥和侧副叶以及不成对的中央体。视叶位于前脑的两侧后端。蕈形体位于脑半球正中间位置。侧副叶在中央体的下前方两侧。中央体在脑的正中心。前脑桥在中央体的上方后侧。除这些形态结构明显的神经髓区域外,前脑还包括大量内部边界不明显的神经髓区域,位于前脑左右两侧以及背侧和腹侧,这些区域被总称为中间脑,占整个脑神经髓的66%。触角叶为中脑的主要组成部分,在脑的下部最前端,为一对球状结构。后脑在脑的腹侧和触角叶下方,即围咽神经索进入脑的入口处。【结论】构建了茶尺蠖5龄幼虫脑以及各神经髓结构三维模型,分析了脑内各个神经髓之间的空间位置关系,明确了各神经髓的体积。茶尺蠖幼虫脑体积小而且结构简单的特征与其幼期视觉、嗅觉等感觉器官不发达、活动能力弱、行为简单的生物学习性相对应。
【Objective】 The purpose of this study was to determine the brain anatomy of the 5th instar larvae of Ectropis obliqua Prout and to analyze and construct the three-dimensional structure models of larval brain and intraneuronal structures. 【Methods】 Immunohistochemistry was used to detect the structure of neurons in the brain by synaptic protein antibody staining and staining of brain synapses. Laser scanning confocal microscopy was used to acquire the brain scan images. Then the images were analyzed by three-dimensional image analysis software AMIRA to construct the three-dimensional brain structure model and calculate the volumes of brain and nerve structure in each brain. 【Result】 The results of SDS-PAGE showed that there were many neuronal synaptic connections in the brain of the 5th instar larvae. These different regions were different neuromuscular structures of the brain. Tea larvae larval brain mainly includes three components of the forebrain, midbrain and hindbrain. Forebrain largest, including the paired leaf lobe, mushroom body, anterior pons and lateral accessory leaves, and unpaired central body. Optic lobes are located at the posterior end of both sides of the forebrain. Mushroom shape is located in the middle of the cerebral hemisphere. Side of the leaves in the central front of the lower body on both sides. The central body is at the very center of the brain. The anterior pons are above the posterior side of the central body. In addition to these morphologically distinct neuromuscular regions, the forebrain also includes a large number of neuromuscular regions with insignificant internal boundaries located on the left and right forebrain as well as the dorsal and ventral regions, collectively referred to as the midbrain, 66% of the marrow. The antennal lobe is a major component of the midbrain, the foremost part of the lower part of the brain, a pair of globular structures. Hindbrain in the ventral and tentorial lobes of the brain, that is, perineal nerve cable into the brain at the entrance. 【Conclusion】 The brain of 5th instar larvae and the three-dimensional model of each neuromuscular structure were constructed. The spatial relationship among each nerve pulp in the brain was analyzed, and the volume of each nerve marrow was clarified. The larval brain is small in size and simple in structure, and its larval brain is characterized by its underdeveloped sense of vision and sense of smell, weak biological activity, and simple biological behavior.